摘要：In the present study,effect of different regen-eration media (R) and combination (R1 = MS me-dium,R2 = MS Salts + B5 vitamins,R3 = MS salts + SH vitamins,R4 = SH medium,R5 = SH salts + MS vitamins,R6 = SH salts + B5 vitamins) and various carbon sources (glucose,sucrose and fruc-tose) was investigated on efficient in vitro shoot multiplication of Riccia fluitans.The media compo-sition significantly affected (P < 0.05) length,width,area,fresh and dry weight of clumps on multiplied plants.The R5 medium produced highest length (2.58 cm),width (2.16 cm),area (5.57 cm2),fresh weight (402.6 g) and dry weight (37.6 g) on regenerated clumps that showed healthier and green plant on culture medium.However,the regenerated clumps diameter was also significantly influenced (P < 0.05) by the type and concentration of sugar (carbon sources) used.The highest clump diameter (7.27 cm) was obtained on R5 medium supplement-ed with 1.5% sucrose.Whereas,the higher concen-tration fructose (4.5 and 6.0%) showed abnormali-ties and phenotypic growth inhibition on cultured explants.The present paper reports direct produc-tion of multiple shoots of riccia plant make it a potential system for developing true-to-type plant that maintain genetic stability for future commercial production of this ornamental aquatic plant.
关键词：Aquatic plant;acclimatization;carbon source;micropropagation;Riccia fluitans L.
