摘要：Meloidogyne graminicola is one of the plant parasitic nematodes that affect rice yield most.In recent years,the occurrence area of rice root-knot nematode disease in rice growing areas in China has been increasing year by year,becoming a major root disease that restricts rice quality and yield.Destruction of neuropeptide signals of nematodes can disrupt their behavior and is one of the effective ways to control nematodes populations.In this study,M.graminicola was used as the object,and the simple collected from the field in Hainan is-landwere identified.Four flp genes of M.graminicola were cloned and their functions were analyzed by in situ hybridization mapping and RNAi technology.The population 1MSY was identified as M.graminicola by morphological observation,molecular biological technology and homology analysis; four flp genes of M.graminicola,including Mg-flp-1 (GenBank accession number MK886770),Mg-flp-13 (GenBank accession number MK886771),Mg-flp-14 and Mg-flp-18 fragments were cloned by RT-PCR,and their fragment sizes were 493 bp,269 bp,673 bp,721 bp respectively.The full length of Mg-flp-14 gene 1,047 bp (GenBank accession number MK855119) and the Mg-flp-18 gene's 3'end sequence 931 bp (GenBank accession number MK886769) were amplified by RACE technology.Mapping analysis of Mg-flp-1,Mg-flp-13,Mg-flp-14,and Mg-flp-18 genes of M.graminicola was carried out by in situ hybridization; RNAi technology was used to detect the silencing effect of Mg-flp-1,Mg-flp-13,Mg-flp-14 and Mg-flp-18 genes.Flp gene has the potential to control broad-spectrum nematodes to manage root-knot nematode disease in rice field planting system.
