标题：Cholesterol Reduction and Vitamin B<sub>12</sub> Production Study on <i>Enterococcus faecium</i> and <i>Lactobacillus pentosus</i> Isolated from Yoghurt
摘要：The present study was aimed to test cholesterol reduction and vitamin B<sub>12</sub> production abilities of the isolated lactic acid bacteria (LAB). Three LAB isolates, namely, <i>Enterococcus faecium</i> (EF), <i>Enterococcus faecium</i> (Chole1), and <i>Lactobacillus pentosus</i> (7MP), having probiotic potential, were isolated from yoghurt. These isolates were screened for bile salt hydrolase (BSH) activity, cholesterol reduction property in MRS broth, and the production of vitamin B<sub>12</sub>. The present study revealed that the isolate 7MP possesses the highest potential of (48%) cholesterol reduction compared to the other isolates. The isolates EF and Chole1 produced a good amount of (1 ng/mL) vitamin B<sub>12</sub>. These isolates were identified by 16S rRNA gene sequencing and confirmed by MALD_TOF analysis. Thus, the use of these LAB isolates for yoghurt-making can offer the value addition of lowering cholesterol and vitamin B<sub>12</sub> fortification in fermented food.
