摘要：HLA class II (HLA-II) genes’ polymorphism influences the immune response to
Chlamydia trachomatis (
Ct), it is considered a sexually transmitted infection. However, associations between HLA-II alleles and
Ct-infection have been little explored in humans; this study was thus aimed at determining HLA-
DRB1-
DQB1 alleles/haplotypes’ effect on
Ct-infection outcome in a cohort of Colombian women. Cervical sample DNA was used as template for detecting
Ct by PCR and typing HLA-
DRB1-
DQB1 alleles/haplotypes by Illumina MiSeq sequencing. Survival models were adjusted for identifying the alleles/haplotypes’ effect on
Ct-outcome; bioinformatics tools were used for predicting secreted bacterial protein T- and B-cell epitopes. Sixteen HLA-
DRB1 alleles having a significant effect on
Ct-outcome were identified in the 262 women analysed.
DRB1*08:02:01G and
DRB1*12:01:01G were related to infection-promoting events. Only the
DQB1*05:03:01G allele related to clearance/persistence events was found for HLA-
DQB1. HLA-
DRB1 allele homozygous women were associated with events having a lower probability of clearance and/or early occurrence of persistence. Twenty-seven peptides predicted in silico were associated with protective immunity against
Ct; outer membrane and polymorphic membrane protein-derived peptides had regions having dual potential for being T- or B-cell epitopes. This article describes HLA-
DRB1-
DQB1 alleles/haplotypes related to
Ct-infection resolution and the peptides predicted in silico which might probably be involved in host immune response. The data provides base information for developing future studies leading to the development of effective prevention measures against
Ct-infection.
