摘要：SummaryAdvancing age has a negative impact on female fertility. As implantation rates decline during the normal maternal life course, age-related, embryonic factors are altered and our inability to monitor these factors in an unbiased genome-wide mannerin vivohas severely limited our understanding of early human embryo development and implantation. Our high-throughput methodology uses trophectoderm samples representing the full spectrum of maternal reproductive ages with embryo implantation potential examined in relation to trophectoderm transcriptome dynamics and reproductive maternal age. Potential embryo-endometrial interactions were tested using trophectoderm sampled from young women, with the receptive uterine environment representing the most ‘fertile’ environment for successful embryo implantation. Potential roles for extracellular exosomes, embryonic metabolism and regulation of apoptosis were revealed. These biomarkers are consistent with embryo-endometrial crosstalk/developmental competency, serving as a mediator for successful implantation. Our data opens the door to developing a diagnostic test for predicting implantation success in women undergoing fertility treatment.Graphical abstractDisplay OmittedHighlights•Trophectoderm transcriptome profiles are altered with maternal age•Trophectoderm transcriptome profiles can delineate maternal reproductive biological age•A genome-wide methodology reveals potential embryo-endometrial communication•Exosome-related expression that could mediate implantation success decreases with agingEmbryology; Omics
