摘要：SummaryLeucine-rich repeat receptor-like kinases (LRR-RLKs) are critical signal receptors in plant development and defense. Like plants, oomycete pathogen genomes also harbor LRR-RLKs, but their functions remain largely unknown. Here, we systematically characterize all the 24LRR-RLKgenes (PsRLKs) fromPhytophthora sojae, which is a model of oomycete pathogens. Although none of them was required for vegetative growth, the specific PsRLKs are important for stress responses, zoospore production, zoospores chemotaxis, and pathogenicity. Interestingly, the Gα subunit PsGPA1 interacts with the five chemotaxis-related PsRLKs via their intracellular kinase domains, and expression ofPsGPA1gene is downregulated in the three mutants (ΔPsRLK17/22/24). Moreover, we generated the PsRLK-PsRLK interaction network ofP. sojaeand found that PsRLK21, together with PsRLK10 or PsRLK17, regulate virulence by direct association. Taken together, our results reveal the diverse roles of LRR-RLKs in modulatingP. sojaedevelopment, interaction with soybean, and responses to diverse environmental factors.Graphical abstractDisplay OmittedHighlights•Systematically functional analysis of LRR-RLK family with 24 members inP. sojae•Five chemotaxis-related PsRLKs directly interact with Gα protein PsGPA1•PsRLKs form an interaction network inP. sojae•The complex PsRLK21-PsRLK10/17 jointly regulates pathogenesisBiological sciences; Microbiology; Plant biology
