摘要：AbstractBacteriocin-producingBacillusspecies have an attractive interest as less studied spore-forming probiotics for food production. In this study, 20 strains ofBacilluswere isolated fromsoumbala, a local fermented food condiment from Burkina Faso. They were characterized based on phenotype, antibacterial activity againstListeria monocytogenesNTCT983 and genotypic analysis by PCR, using specific primers forBacillussp.,B. subtilisgroup,B. cereusgroup, bacteriocin genes, subtilin gene (spaS) and subtilosin gene (sboA). All the isolates were presumptively identified asBacillusspp. with predominance ofB. subtilisspecies (30%). Out of 20 strains, 11 showed anti-Listeriaactivity, with inhibition zones ranging from 11.12±0.53 mm (strain B21) to 25.25±0.35 mm (strain F25). PCR profiles revealed that 18 strains belonged to theB. subtilisgroup while O28 and O46 belonged to theB. cereusspecies. In addition, 13 strains carried bacteriocin genes, 10 possessed genesboAand only strain O48 carried genespaS. This finding highlights the predominance of bacteriocin-producingB. subtilisgroup species inSoumbalaproduction population. These could be further characterized as probiotic starter cultures carrier for controlled production of high-qualitysoumbala.
