摘要：The work reported in this article raises some serious concern about the drinking water quality and its standards. Mere presence or absence of an indicator organism does not assure that the water is safe for drinking purposes. Instead of infecting directly, many pathogens pass through a host and retrieve their virulent properties by causing diseases/infections in humans. Pathogenic bacteria which exist in aquatic habitats show a unique and peculiar pattern of appearing or reappearing in different microenvironments. Several factors that prevail in the water system make a safe house for the growth, proliferation, and colonization of microorganisms. In our case, 6 different microenvironments inside the premises of an office building were taken as the sampling sites to study the effect of seasonal variations (summer, monsoon, and post-monsoon/winter) on bacterial diversity and inhabitants. Results suggested that the presence of total and thermotolerant coliforms were highest in the monsoon followed by summer and post-monsoon/winter seasons. To know the bacterial diversity and pattern of appearance/reappearance prevailing in the water system, bacterial strains were analyzed by 16S rRNA sequencing which showed Pseudomonas putida to be the predominant identified bacterial strain occurring about 38% to 77% in all 3 seasons. This was followed by Lelliottia nimipressuralis (6%-21%), Escherichia coli (4%-18%), Salmonella typhimurium and Aeromonas dhakensis (4%-10% each), and Klebsiella pneumoniae (5%-6%). Despite the absence of other opportunistic bacteria, P putida was reported to be present as a single organism in water coolers and dispensers. This might be due to the persistent nature of P putida in low-nutrient environments and capable of colonizing by forming a rigid biofilm inside the water cooler/dispenser which makes a conducive environment for it.
