期刊名称：Acta Universitatis Cibiniensis. Series E: Food Technology
印刷版ISSN：2344-150X
出版年度：2021
卷号：25
期号：1
页码：135-142
DOI：10.2478/aucft-2021-0013
语种：English
出版社：Walter de Gruyter GmbH
摘要：Cell-envelope proteinases (CEPs) can hydrolyze casein into functional peptides, which is beneficial to the health of the host. The single factor experiment screened out that the optimal conditions for CEP production by Lactobacillus plantarum LP69 were 37°C, 20h, initial pH of 7, and optimal inoculation amount of 5%. The best conditions for this experiment were obtained by orthogonal experiment: time 22h, temperature 39°C, initial pH value of 6, and inoculation amount of 5%. Under this culture condition, the target protease activity of Lactobacillus plantarum LP69 reached 22.31±0.82U/mL, the protein content was 19.07±0.36mg/mL, and the specific activity was 1.17±0.06U/mg. The specific activity significantly increased by 15.8% compared with the control (p0.05).
