摘要：SummaryThe mouse eye is used to model central nervous system development, pathology, angiogenesis, tumorigenesis, and regenerative therapies. To facilitate the analysis of these processes, we developed an optimized tissue clearing and depigmentation protocol, termedInVision, that permits whole-eye fluorescent marker tissue imaging. We validated this method for the analysis of normal and degenerative retinal architecture, transgenic fluorescent reporter expression, immunostaining and three-dimensional volumetric (3DV) analysis of retinoblastoma and angiogenesis. We also used this method to characterize material transfer (MT), a recently described phenomenon of horizontal protein exchange that occurs between transplanted and recipient photoreceptors. 3D spatial distribution analysis of MT in transplanted retinas suggests that MT of cytoplasmic GFP between photoreceptors is mediated by short-range, proximity-dependent cellular interactions. TheInVisionprotocol will allow investigators working across multiple cell biological disciplines to generate novel insights into the local cellular networks involved in cell biological processes in the eye.Graphical abstractDisplay OmittedHighlights•InVisionis an optimized tissue clearing protocol for the rodent eye•InVisioncan be used to study a wide variety of physiological processes in the eye•Material transfer between transplanted and host photoreceptors is spatially correlatedOptical imaging; Biological sciences; Neuroscience; Biotechnology; Biological sciences research methodologies; Biology experimental methods
