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  • 标题:Preliminary Evaluation of Lectins as Fluorescent Probes of Seed Structure and Composition
  • 本地全文:下载
  • 作者:Miller, S. Shea ; Yiu, S. H. ; Fulcher, R. G.
  • 期刊名称:Food Structure
  • 出版年度:1984
  • 卷号:3
  • 期号:2
  • 页码:5
  • 出版社:Utah State University
  • 摘要:Several commercially available fluoresceinisothiocyanate and rhodamine isothiocyanateconjugated plant lectins have been applied to cereal and oilseed tissues to permit identification and localization of specific structures and carbohydrates by fluorescence microscopy . Ulex europeaus Agglutinin I (UEAl) and Ricinis communis Agglutinin I (RCA I) showed specificity for the amyloids in rapeseed cotyledonary cell walls . Wheat Germ Agglutinin (WGA) bound to rapeseed coat mucilage, as well as fungal hyphae in infected wheat . Lens culinaris Agglutinin (LCA) bound only to starch in cereal sections, and at higher magnifications of isolated starch granules , the annular structure was clearly visible.
  • 关键词:Lectins; fluorescence microscopy; seed structure; cell walls; carbohydrates; amyloid; mucilage
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