摘要:This research includes the analysis and comparison of long-term values of key business parameters of profit-oriented companies in Serbia, which are engaged in road transport of cargo or passengers. This paper takes into account the decreasing emissions of CO<sub>2</sub> and its relation to the size of business (in terms of transported cargo or number of passengers), and thus by the company’s business success (income, profit). In the empirical part of this research—ecological, operational, and business factors were analyzed on a sample of road carriers from Serbia, i.e., the most common type of organized transport of people or physical goods. Key difference was made between large and small companies engaged in transport activities, followed by difference between those companies which have business activities only in Serbia, or engage also in international activities in the Balkan region (or in the rest of world). The main goal of this paper is to determine statistically significant differences between transport companies in terms of key performance indicators, depending on whether they operate only domestically or abroad. In relation to company size, this paper examined the sustainability of operations in the case of the largest transport companies, which represent half of the total transport activity in the country (by number of people transported or the amount of transported cargo), compared to all small carriers with less than 50 employees. Future research involves extending this sample of road transport companies to all Balkan countries, which have not yet become a part of the European Union and including additional operational as well as environmental indicators that are not conventionally measured during vehicle inspections.