期刊名称：Journal of Advanced Veterinary and Animal Research
印刷版ISSN：2311-7710
出版年度：2021
卷号：8
期号：2
页码：274-281
DOI：10.5455/javar.2021.h512
语种：English
出版社：Bangladesh Agricultural University, Faculty of Veterinary Science
摘要：Objective:
Fast foods are often responsible for staphylococcal foodborne illness. The present study was carried out to isolate
Staphylococcus spp. from various fast foods sold in Mymensingh and to determine their antibiogram.
Materials and Methods:
Overall, 60 samples of fast foods sold in different restaurants were screened by culture, biochemical tests, and polymerase chain reaction (PCR) to isolate and identify
Staphylococcus spp., followed by employing of disk diffusion method to determine their antibiotic resistance patterns.
Results:
Among these 60 samples, 8 [13.33%, 95% confidence interval (CI): 6.91%–24.17%] were positive for
Staphylococcus spp. by cultural and biochemical properties. By PCR, four (6.67%, 95% CI: 2.62%–15.93%) isolates were confirmed as
Staphylococcus aureusby targeting
nuc gene. Additionally, all the
S. aureus isolates were coagulase-positive. By antibiogram profiles, all the
Staphylococcus isolates exhibited resistance to azithromycin and erythromycin (95% CI: 67.56%–100.00%), and frequently resistance to cefixime (75%, 95% CI: 40.93%–95.56%), ampicillin (50%, 95% CI: 21.52%–78.48%), and amoxicillin (37.5%, 95% CI: 13.68%–69.43%); moderate to lower resistance was found against cefotaxime, gentamicin, and doxycycline. In addition, all the isolates were sensitive to ciprofloxacin and chloramphenicol. Interestingly, 75% (6/8; 95% CI: 40.93%–95.56%) isolates were multidrug-resistant (MDR) in nature. Furthermore, the indices of multiple antibiotic resistance (MAR) were ranged from 0.2 to 0.6.
Conclusion:
This study revealed that fast foods sold in restaurants were contaminated with MDR and MAR
Staphylococcus isolates, having potential public health significance.
