摘要：Heat shock proteins contribute to diabetes-induced complications and are affected by glycemic control. Our hypothesis was that hypoglycemia-induced heat shock and related protein changes would be amplified in type 2 diabetes (T2D). This prospective, case–control study enrolled 23 T2D patients and 23 control subjects who underwent hyperinsulinemic-induced hypoglycemia (≤ 2.0 mmol/L (36 mg/dl)) with blood sampling at baseline, at hypoglycemia and after a 24-h post-hypoglycemia follow-up period. Proteomic analysis of heat shock-related and pro-inflammatory proteins was performed. At baseline, MAPKAPK5 (
p = 0.02) and UBE2G2 (
p = 0.003) were elevated and STUB1 decreased (
p = 0.007) in T2D. At hypoglycemia: PPP3CA (
p < 0.03) was increased and EPHA2 (
p = 0.01) reduced in T2D; by contrast, three proteins were reduced in controls [HSPA1A (
p = 0.007), HSPB1 (
p < 0.02), SMAD3 (
p = 0.005)
