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  • 标题:Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application
  • 本地全文:下载
  • 作者:Narges Asefifeyzabadi ; Torrey E. Holland ; Poopalasingam Sivakumar
  • 期刊名称:Biosensors
  • 电子版ISSN:2079-6374
  • 出版年度:2021
  • 卷号:11
  • 期号:8
  • DOI:10.3390/bios11080273
  • 语种:English
  • 出版社:MDPI Publishing
  • 摘要:DNA is strongly adsorbed on oxidized graphene surfaces in the presence of divalent cations. Here, we studied the effect of DNA adsorption on electrochemical charge transfer at few-layered, oxygen-functionalized graphene (GO x) electrodes. DNA adsorption on the inkjet-printed GO x electrodes caused amplified current response from ferro/ferricyanide redox probe at concentration range 1 aM–10 nM in differential pulse voltammetry. We studied a number of variables that may affect the current response of the interface: sequence type, conformation, concentration, length, and ionic strength. Later, we showed a proof-of-concept DNA biosensing application, which is free from chemical immobilization of the probe and sensitive at attomolar concentration regime. We propose that GO x electrodes promise a low-cost solution to fabricate a highly sensitive platform for label-free and chemisorption-free DNA biosensing.
  • 关键词:enDNA biosensors;graphene electrodes;inkjet-printing;trinucleotide repeats;label-free;electrochemical biosensors
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