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  • 标题:Author Correction: Lgals9 deficiency ameliorates obesity by modulating redox state of PRDX2
  • 本地全文:下载
  • 作者:Tomokazu Nunoue ; Satoshi Yamaguchi ; Sanae Teshigawara
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2021
  • 卷号:11
  • DOI:10.1038/s41598-021-98293-1
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Correction to: Scientific Reports 10.1038/s41598-021-85080-1, published online 16 March 2021 The original version of this Article contained errors. In Figure 5, the size marker gel image was mistakenly placed in Panel (e). The original Figure  5 and accompanying legend appear below. Figure 5 Pull-down assay and Gal-9 siRNA experiments in 3T3L1 cells. ( a) PRDX2-FLAG-HA-pcDNA3.1 (PRDX2) and FLAG-HA-pcDNA3.1 (HA) were transfected into 3T3L1 cells. In the presence of 0.2 M lactose, the protein complexes were HA-tag purified with Anti-HA tag Beads, and subjected to SDS-PAGE under reducing conditions and Western blot analysis. The membrane was incubated with anti-Gal-9 antibody. ( b) The membrane was stripped off and incubated with anti-peroxiredoxin 2 (PRDX2) antibody. ( c) The membranes was again stripped off and incubated with anti-thioredoxin (TRX) antibody. ( d) 3T3L1 cells were treated with Silencer select Pre-designed siRNA Lgals9 (Gal-9 siRNA) and Silencer select negative control siRNA (NC siRNA) for 40 h. ( e) After the treatment of 3T3L1 cells with siRNAs, the cells were further cultured in the absence and presence of 10 μM H 2O 2 for 20 min. *,  p  < 0.05; **,  p  < 0.01. Two-pair comparisons by Student’s  t test. Additionally in Supplementary Figure 4 and 5, the panel labels and corresponding red boxes for uncropped images were omitted. The original Supplementary Information 1 file is provided below. The original Article and accompanying Supplementary Information 1 file have been corrected. Supplementary Information Supplementary Information 1.
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