摘要：<p class="MsoNormal" style="margin-bottom: 0,0000pt; text-align: justify; text-justify: inter-ideograph;" align="justify"><strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; mso-ansi-font-weight: bold; font-size: 12,0000pt;">Background.</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> Earlier we found that even in intact rats, certain parameters of nitrogenous metabolism fluctuate in a fairly wide range, which further expands in cases of prolonged water loads.</span><span class="16" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">Based on this, </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">we</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> have been created three groups that are homogeneous in the parameters of nitrogenous metabolism. We have been shown that each cluster is accompanied by a specific constellation of immune parameters. In this study, an attempt will be made to supplement the immune accompaniment of each constellation of nitrogenous metabolites with parameters of the autonomic nervous and endocrine systems. </span><strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; mso-ansi-font-weight: bold; font-size: 12,0000pt;">Material and methods</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">. </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">Experiment was performed on </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">60</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> healthy female Wistar rats</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">, both intact and loaded with different mineral waters</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">. </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">Immune status was assessed by thymocytogram, splenocytogram, blood leukocytogram and immunocytogram, as well as by phagocytosis parameters of blood neutrophils and monocytes.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> The state of autonomous regulation assessed by HRV. The plasma levels of the hormones of adaptation: corticosterone, triiodothyronine and testosterone (by the </span><span class="17" style="font-family: 'Times New Roman'; color: #222222; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">ELISA</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">) were determined as well as mineralocorticoid, calcitonin and parathyroid activity calculated by</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> their electrolyte markers</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">.</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> </span><strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; mso-ansi-font-weight: bold; font-size: 12,0000pt;">Results</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">. </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">9</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> neuro-endocrine and</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> 17</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> immune parameters were </span><span class="15" style="font-family: 'Times New Roman';">identified, the set of which three clusters of nitrogen metabolism clearly differ from each other</span><span class="15" style="font-family: 'Times New Roman';">. </span><span class="15" style="font-family: 'Times New Roman';">The overall accuracy of the classification is 9</span><span class="15" style="font-family: 'Times New Roman';">3,3</span><span class="15" style="font-family: 'Times New Roman';">%.</span><span style="font-family: 'Times New Roman';"> </span><strong><span class="15" style="font-family: 'Times New Roman';">Conclusion</span></strong><span class="15" style="font-family: 'Times New Roman';">. The variety of states of </span><span class="15" style="font-family: 'Times New Roman';">exchange of </span><span class="15" style="font-family: 'Times New Roman';">nitrogenous metabolites is accompanied by specific constellations of 26 parameters of neuro-endocrine-immune complex.</span></p>
