摘要：<p class="MsoNormal"><strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; mso-ansi-font-weight: bold; font-size: 12,0000pt;">Background.</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> Earlier we found that even in intact rats, certain parameters of nitrogenous metabolism and immunity fluctuate in a fairly wide range, which further expands in cases of prolonged water loads.</span><span class="16" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">The links between the individual parameters of metabolism and immunity were revealed.</span><span class="16" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">Based on this, the </span><strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; mso-ansi-font-weight: bold; font-size: 12,0000pt;">aim </span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">of this study was to create groups that are homogeneous in the parameters of nitrogenous metabolism, followed by a search for the characteristic parameters of immunity. </span><strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; mso-ansi-font-weight: bold; font-size: 12,0000pt;">Material and methods</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">. </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">Experiment was performed on </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">60</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> healthy female Wistar rats</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">, both intact and loaded with different mineral waters</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">. The plasma levels and urinary excretion of the nitrogenous metabolites </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">(creatinine, urea, uric acid and bilirubin) </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">were determined</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">. </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">Immune status was assessed by thymocytogram, splenocytogram, blood leukocytogram and immunocytogram, as well as by phagocytosis parameters of blood neutrophils and monocytes.</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> </span><strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; mso-ansi-font-weight: bold; font-size: 12,0000pt;">Results</span></strong><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">. </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">The characteristic features of the members of the most numerous (29 animals) of the first cluster are moderately elevated plasma urea and upper borderline creatinineemia in combination with lower borderline uricemia. In members of the third cluster (22 animals), all seven parameters are in the range of normal.</span><span class="16" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">In contrast, rats of the second cluster (n=9) were found to have significantly increased levels of urea and creatinine excretion, as well as uricemia.</span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">Each cluster is accompanied by a specific constellation of immune parameters.</span><span class="16" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;"> </span><span class="15" style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; mso-fareast-font-family: Calibri; font-size: 12,0000pt;">The overall accuracy of the </span><span class="15" style="font-family: 'Times New Roman';">classification is 95%.</span><span style="font-family: 'Times New Roman';"> </span><strong><span class="15" style="font-family: 'Times New Roman';">Conclusion</span></strong><span class="15" style="font-family: 'Times New Roman';">. The variety of states of </span><span class="15" style="font-family: 'Times New Roman';">exchange of </span><span class="15" style="font-family: 'Times New Roman';">nitrogenous metabolites is accompanied by specific constellations of immune parameters.</span></p>
