摘要：SummaryThe paraventricular nucleus of the thalamus (PVT) controls goal-oriented behavior through its connections to the nucleus accumbens (NAc). We previously characterized Glut2aPVTneurons that are activated by hypoglycemia, and which increase sucrose seeking behavior through their glutamatergic projections to the NAc. Here, we identified glucokinase (Gck)-expressing neurons of the PVT (GckaPVT) and generated a mouse line expressing the Cre recombinase from the glucokinase locus (GckCre/+mice).Ex vivocalcium imaging and whole-cell patch clamp recordings revealed that GckaPVTneurons that project to the NAc were mostly activated by hyperglycemia. Their chemogenetic inhibition or optogenetic stimulation, respectively, enhanced food intake or decreased sucrose-seeking behavior. Collectively, our results describe a neuronal population of Gck-expressing neurons in the PVT, which has opposite glucose sensing properties and control over feeding behavior than the previously characterized Glut2aPVTneurons. This study allows a better understanding of the complex regulation of feeding behavior by the PVT.Graphical abstractDisplay OmittedHighlights•Glucokinase (Gck) is expressed in neurons of the paraventricular thalamus (PVT)•Most Gck-expressing PVT neurons are glucose-excited neurons•Chemogenetic inhibition of the GckPVTneurons increases food intake•Optogenetic activation of accumbens-projecting GckPVTneurons decreases sucrose seekingBehavioral neuroscience; Cellular neuroscience; Neuroscience
