摘要：SummaryFeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields to activate the transient receptor potential channels, TRPV1 and TRPV4, coupled to cellular ferritins. In cells expressing ferritin-tagged TRPV, RF stimulation increases the cytosolic Ca2+levels via a biochemical pathway. The interaction between RF and ferritin increases the free cytosolic iron levels that, in turn, trigger chemical reactions producing reactive oxygen species and oxidized lipids that activate the ferritin-tagged TRPV. In this pathway, it is expected that experimental factors that disturb the ferritin expression, the ferritin iron load, the TRPV functional expression, or the cellular redox state will impact the efficiency of RF in activating ferritin-tagged TRPV. Here, we examined several experimental factors that either enhance or abolish the RF control of ferritin-tagged TRPV. The findings may help optimize and establish reproducible magnetogenetic protocols.Graphical abstractDisplay OmittedHighlights•Diverse experimental factors affect the efficiency of magnetogenetics•Efficacy of magnetogenetics is affected by the downregulation of TRPV4 channels•The temperature-insensitive TRPV4 mutant is suitable for use in magnetogenetics•Ferritin iron load impacts the magnetic activation of ferritin-tagged TRPV4Bioelectromagnetics; Genetics; Biology experimental methods
