摘要：Multiplications, mutations and dysregulation of the alpha synuclein gene (
SNCA) are associated with the demise of dopaminergic neurons and are considered to play important roles in the pathogenesis of familial and sporadic forms of Parkinson’s disease. Regulation of
SNCA expression might thus be an appropriate target for treatment. We aimed to identify specific modulators of
SNCA transcription, generated CRISPR/Cas9 modified
SNCA-
GFP-luciferase (
LUC) genomic fusion- and control cell lines and screened a library of 1649 bioactive compounds, including the FDA approved drugs. We found no inhibitors but three selective activators which increased
SNCA mRNA and protein levels.
