摘要：For sensitive and fast detection of
Escherichia coli O157:H7, organic and inorganic hybrid Au/Fe
3+ nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe
3+ porous spongy NCs with large surface area showed excellent bio-specific capability for
E. coli O157:H7. GNPs in Au/Fe
3+ NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe
3+ NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of
E. coli O157:H7, which showed a good linear response (10
1 to 10
6 cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60–97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of
E. coli O157:H7.
