标题：Isolation, Characterization and Identification of a New <i>Lysinibacillus fusiformis</i> Strain ZC from Metlaoui Phosphate Laundries Wastewater: Bio-Treatment Assays
摘要：The aim of the present study is to isolate, characterize and identify a novel strain ZC from the Metlaoui phosphate laundries wastewater (MPLW). The chemical characterization of this phosphate rich effluent showed an alkaline pH and is saline, highly turbid and rich in suspended matter and total solids. The MPLW samples were loaded with potentially toxic metals, presented in decreasing order as follows: magnesium (5655 mg L<sup>−1</sup>), potassium (45 mg L<sup>−1</sup>), lead (1 mg L<sup>−1</sup>), iron (0.7 mg L<sup>−1</sup>), cadmium (0.5 mg L<sup>−1</sup>), copper (0.3 mg L<sup>−1</sup>) and zinc (0.1 mg L<sup>−1</sup>). Due to the high COD/BOD<sub>5</sub> ratio, a poorly biodegradable organic load is underlining. The newly isolated strain was identified as <i>Lysinibacillus fusiformis</i> using 16S rDNA sequencing analysis. The viability of this new strain was tested in presence of the zinc, lead, cadmium, manganese and copper at 1, 10 and 100 mM. The <i>L. fusiformis</i> survival, under metallic stress, was inversely proportional to metal ion concentrations, while lead and zinc were the most toxic ones using MTT assay. Then, the newly isolated strain was characterized in terms of enzyme production, proteomic alteration and antibiotic resistance. The strain ZC revealed some modifications in the biochemical and enzymatic profiles by either the appearance or/and the disappearance of some activities. In addition, the increase in metal ions stress and concentrations was proportional to the adherence and to the hydrophobicity. The presence of the metal ions suggested the change of sensitivity to the resistance of this strain towards tobramycin, kanamycin, neomycin, netilmicin and cefoxitin, showing an increase in the MAR<sub>index</sub>. The strain ZC, used as a biological tool for MPLW treatment, showed a reduction in the metal ion contents. This reduction was due to accumulation and/or adsorption, showing a bioprocessing performance of the newly isolated <i>L. fusiformis</i>.
