标题：Antifungal Activity of Earthworm Coelomic Fluid Obtained from Eisenia andrei, Dendrobaena veneta and Allolobophora chlorotica on Six Species of Phytopathogenic Fungi
摘要：The functioning of soil ecosystems greatly depends on the interactions occurring between soil biota communities. It is well known that earthworms are an important soil component that substantially affects its function, including their meaningful impact on the development of different phytopathogenic soil fungi. Phytopathogenic fungi are responsible for crop disease and cause great economic damage. It has previously been established that earthworms’ coelomic fluid can suppress the growth of phytopathogenic fungi, but the exact molecular mechanism is unknown. The present study aimed at broadening the proof of this observed phenomenon by investigating the effects of the coelomic fluid extract of three different earthworm species (<i>Eisenia andrei</i>, <i>Dendrobaena veneta</i> and <i>Allolobophora chlorotica</i>) on the growth of six different phytopathogenic fungi species (<i>Berkeleyomyces basicola</i>, <i>Fusarium culmorum</i>, <i>Globisporangium irregulare, Rhizoctonia solani</i>, <i>Macrophomina phaseolina</i>, and <i>Sclerotinia sclerotiorum</i>). Coelomic fluid extract was obtained by electrostimulation or usage of extraction buffer (only in case of <i>A. chlorotica</i>) and prepared in three different concentrations by diluting the obtained coelomic fluid with physiological saline. The coelomic fluid extract of the three investigated earthworm species had an inhibitory effect on the growth of all six phytopathogenic fungi species. The greatest inhibitory effect was achieved with the <i>E. andrei</i> coelomic fluid extract reducing the growth of <i>R. solani</i> fungi. The findings of this research confirm the antifungal activity of coelomic fluid obtained from earthworm species belonging to different ecological categories and may be of potential use in crop protection against phytopathogenic fungi.
