摘要：Background and Objective: Mango peel is an excellent source of bioactive compounds especially Phenolic Compounds (PC). Due to the difficulty of adding the mango peel to food products as a solution we evaluated the feasibility of encapsulating a phenolic-rich extract from mango peel (MPPE) then added to milk beverage and the therapeutic effectiveness in protecting against oxidative stress was investigated. Materials and Methods: The characterization of MPPE microcapsules has been conducted by Scanning Electron Microscopy (SEM) and Encapsulation Efficiency (EE). The MPPE microcapsules were added in different proportions to the milk (1, 2 and 3%), physicochemical properties and the viscosity were evaluated during 14 days of cold storage. In vitro simulation digestion process (oral, gastric and intestinal phase) was evaluated for the total phenolic and flavonoid content (TPC and TFC) and antioxidant activity for both Mango Peel Phenolic Extract (MPPE) microcapsules and milk beverage supplemented with MPPE microcapsules. The milk beverage supplemented with microcapsules was investigated against oxidative stress using CCl4 injected rats. Results: The physicochemical properties of the milk beverage supplemented with microcapsule were not significantly affected compared to the control sample. Furthermore, TPC, TFC and antioxidant activity values were increased significantly (2-3 times) at the end of the simulation digestive process for both MPPE microcapsules and milk beverage supplemented with MPPE microcapsules each individually. In vivo experiment, the total antioxidant and catalase levels were increased and the lipid peroxide, Alanine Transaminase (ALT), Aspartate Transaminase (AST) and Alkaline Phosphatase (ALP) activities were decreased when used milk beverage supplemented with MPPE microcapsules. Conclusion: These results demonstrated that MPPE microcapsules rich in bioactive components can be used as a chemo-preventive agent against oxidative stress disorders in experimental rats.
