摘要：SummaryAdult stem cells and their transit-amplifying progeny alter their proliferation rates to maintain tissue homeostasis. To test how the division rates of stem cells and transit-amplifying progeny affect tissue growth and differentiation, we developed a computation strategy that estimates the average cell-cycle lengths (lifespans) of germline stem cells and their progeny from fixed-tissue demography in theDrosophilatestis. Analysis of the wild-type data using this method indicated that during the germline transit-amplification, the cellular lifespans extend by nearly 1.3-fold after the first division and shrink by about 2-folds after the second division. Cell-autonomous perturbations of the stem cell lifespan accordingly altered the lifespans of successive transit-amplifying stages. Remarkably, almost 2-fold alterations in the lifespans of stem cells and their immediate daughters did not affect the subsequent differentiation. The results indicate that the early germline division rates can adjust the following division rates and the onset of differentiation.Graphical abstractDisplay OmittedHighlights•Prediction of cellular lifespan from the demography of transit-amplifying cells•Lifespans of spermatogonial cells change anomalously during transit-amplification•Anomalous lifespan extension during transit-amplification precedes the onset of Bam•Lifespan changes of early TA stages readjust that of the subsequent stagesCell biology; Stem cells research; Bioinformatics
