摘要：Recessive variants of the
SLC26A4 gene are an important cause of hereditary hearing impairment. Several transgenic mice with different
Slc26a4 variants have been generated. However, none have recapitulated the auditory phenotypes in humans. Of the
SLC26A4 variants identified thus far, the p.T721M variant is of interest, as it appears to confer a more severe pathogenicity than most of the other missense variants, but milder pathogenicity than non-sense and frameshift variants. Using a genotype-driven approach, we established a knock-in mouse model homozygous for p.T721M. To verify the pathogenicity of p.T721M, we generated mice with compound heterozygous variants by intercrossing
Slc26a4
+
/T721M
mice with
Slc26a4
919-2A>
G/919-2A>
G
mice, which segregated the c.919-2A > G variant with abolished
Slc26a4 function. We then performed serial audiological assessments, vestibular evaluations, and inner ear morphological studies. Surprisingly, both
Slc26a4
T721M/T721M
and
Slc26a4
919-2A>
G/T721M
showed normal audiovestibular functions and inner ear morphology, indicating that p.T721M is non-pathogenic in mice and a single p.T721M allele is sufficient to maintain normal inner ear physiology. The evidence together with previous reports on mouse models with
Slc26a4 p.C565Y and p.H723R variants, support our speculation that the absence of audiovestibular phenotypes in these mouse models could be attributed to different protein structures at the C-terminus of human and mouse pendrin.
