期刊名称：Journal of Advanced Veterinary and Animal Research
印刷版ISSN：2311-7710
出版年度：2021
卷号：8
期号：3
页码：489-493
DOI：10.5455/javar.2021.h538
语种：English
出版社：Bangladesh Agricultural University, Faculty of Veterinary Science
摘要：Objectives:
The purpose of this study was to determine the species composition of
Eimeria circulating in Mymensingh district, Bangladesh, using Internal Transcribed Spacer 1 (ITS1) sequences in polymerase chain reaction (PCR) assay.
Materials and Methods:
Coccidian oocysts were isolated and sporulated in a solution containing 2% potassium dichromate from litter slurry collected from 13 commercially active broiler farms in the research region. Genomic DNA was isolated from sporulated oocysts and used to amplify the
Eimeria species-specific
ITS1 gene by PCR amplification. Electrophoresis of 1.5% agarose gel was used to visualize the amplified PCR products.
Results:
In the study samples from Mymensingh district, Bangladesh, the presence of
Eimeria brunetti,
Eimeria acervulina,
Eimeria necatrix,
Eimeria mitis, and
Eimeria tenella was identified.
Conclusions:
The findings of this study may shed light on the zonal approach to chicken coccidiosis control. Additionally, it suggests that ITS1-based PCR might be used in the field to accurately identify
Eimeria species.
