摘要：SummaryStreptomycesspecies have attracted considerable interest as a reservoir of medically important secondary metabolites, which are even diverse and different between strains. Here, we reassess tenStreptomyces venezuelaestrains by presenting the highly resolved classification, using 16S rRNA sequencing, MALDI-TOF MS protein profiling, and whole-genome sequencing. The results revealed that seven of the ten strains were misclassified asS.venezuelaespecies. Secondary metabolite biosynthetic gene cluster (smBGC) mining and targeted LC-MS/MS based metabolite screening ofS. venezuelaeand misclassified strains identified in total 59 secondary metabolites production. In addition, a comparison of pyrrolamide-type antibiotic BGCs of four misclassified strains, followed by functional genomics, revealed thatathv28is critical in the synthesis of the anthelvencin precursor, 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate (ADPC). Our findings illustrate the importance of the accurate classification and better utilization of misclassifiedStreptomycesstrains to discover smBGCs and their secondary metabolite products.Graphical abstractDisplay OmittedHighlights•Seven out of tenStreptomyces venezuelaestrains were misclassified•59secondary metabolites productions were identified from the ten strains•Athv28 converts glutamine to ADPC, the most important anthelvencin precursorMicrobiology; Microbial genomics; Phylogeny; Metabolomics
