摘要：SummaryThe microbiota ofAnophelesmosquitoes influences malaria transmission. Antibiotics ingested during a blood meal impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota.We collectedAnopheleslarvae in Burkina Faso, kept them in semi-field conditions, and offered a blood meal to adult females. We tested the impact of blood supplementation with two alternative amoxicillin preparations on microbiota composition, determined by high-throughput sequencing in individual gut samples. Our analysis detected four major genera,Elizabethkingia,Wigglesworthia,Asaia,andSerratia. The antibiotic treatment significantly affected overall microbiota composition, with a specific decrease in the relative abundance ofElizabethkingiaandAsaiaduring blood digestion. Besides its interest on the influence of amoxicillin on the mosquito microbiota, our study proposes a thorough approach to report negative-control data of high-throughput sequencing studies on samples with a reduced microbial load.Graphical abstractDisplay OmittedHighlights•We sequenced bacterial 16S in mosquitoes collected as larvae in Burkina Faso•Elizabethkingia,Wigglesworthia,Asaia, andSerratiadominate the gut microbiota•Amoxicillin treatment and potentially gametocyte presence influence the microbiota•We set up methods to consider mock controls after sequencing low-biomass samplesEntomology; Microbiome
