摘要：SummaryGroup II self-splicing introns are large structured RNAs that remove themselves from transcripts while simultaneously sealing the resulting gaps. Some representatives can subsequently reverse splice into DNA, accounting for their pervasive distribution in bacteria. The catalytically active tertiary structure of each group II intron is assembled from six domains that are arranged in a conserved order. Here, we report structural isomers of group II introns, called CP group II ribozymes, wherein the characteristic order of domains has been altered. Domains five and six, which normally reside at the 3′ end of group II introns, instead occupy the 5′ end to form circularly permuted variants. These unusual group II intron derivatives are catalytically active and generate large linear branched and small circular RNAs, reaction products that are markedly different from those generated by canonical group II introns. The biological role of CP group II ribozymes is currently unknown.Graphical abstractDisplay OmittedHighlights•Circularly permuted versions of group II introns occur in multiple bacterial phyla•The splice sites are reversed in order and located within the interior of the ribozyme•CP group II ribozymes generate large branched and small circular RNA productsin vitro•The biological function of this ribozyme class is not knownBiological sciences; Molecular biology; Structural biology; Bioinformatics
