文章基本信息
- 标题：Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells
- 本地全文：下载
- 作者：Kim, Byung Moon ; Kim, Jae Woo
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2015
- 卷号：56
- 期号：1
- 页码：104-108
- DOI：10.3341/jkos.2015.56.1.104
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effects of benzalkonium chloride (BAC), mitomycin C (MMC) and dexamethasone (DEX) on cellular stress in cultured human trabecular meshwork cell (HTMC) monolayers.
MethodsHTMCs were cultured in the inner Transwell chamber until confluence and then were exposed to BAC, MMC or DEX for 6 hours. The carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer at 532 nm after 2 hours in the outer chamber. The 3-[4, 5 -dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cellular viabilities.
ResultsThe carboxyfluorescein permeability through the HTMC monolayer increased and cell survival decreased with 0.002% BAC ( p < 0.05). Increased permeability without decreasing cell survival occurred with 0.05 µg/mL MMC. No effect on the permeability or cell survival was observed at 0.1 or 1.0 µm DEX ( p > 0.05).
ConclusionsBAC and MMC induced cellular toxicity and stress at lower concentrations but did not affect survival of cultured HTMCs.
- 关键词：Benzalkonium chloride; Carboxyfluorescein; Dexamethasone; Mitomycin C; Trabecular meshwork cells
