摘要：In this study, single nucleotide polymorphisms (SNPs) in the
ANXA9 (annexin 9),
FASN (fatty acid synthase) and
SCD1 (stearoyl-CoA desaturase 1) genes were analyzed as factors influencing fatty acid profiles in milk from Zošľachtená valaška sheep. SNP in selected genes was identified using polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR–RFLP). The long-chain fatty acids profile in sheep milk was identified by gas chromatography. Statistical analysis of the
SCD1/Cfr13I polymorphism showed that the milk of the homozygous
AA animals was characterized by a lower (
P < 0.05) share of C4:0, C6:0, C8:0, C10:0, C12:0, C14:0 in comparison to the homozygous
CC sheep. The milk of heterozygous sheep was characterized by a higher (
P < 0.05) proportion of C13:0 acid compared to the milk of sheep with the homozygous
AA type. A higher (
P < 0.05) level of saturated fatty acids (SFA) was found in the milk of
CC genotype sheep compared to the
AA genotype. Our results lead to the conclusion that the greatest changes were observed for the
SCD1/Cfr13I polymorphism and the least significant ones for
FASN/AciI. Moreover, it is the first evidence that milk from sheep with
SCD1/Cfr13I polymorphism and the homozygous
AA genotype showed the most desirable fatty acids profile.
