期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2021
卷号：69
期号：3
页码：247-255
DOI：10.3164/jcbn.20-185
语种：English
出版社：The Society for Free Radical Research Japan
摘要：Cellular senescence is an intricate and multifactorial phenomenon, which is characterized by an irreversible cellular growth arrest, it is caused in response to irretrievably DNA damage, telomere shorting, activation of oncogene, and oxidative stress. Human diploid fibroblasts are a well-established experimental model for premature senescence-related studies, and exposure of fibroblasts to H
2O
2 is widely used as a SIPS model. Recently, it has been reported many studies of CoQ
10 as to anti-aging effects, however the effect of CoQ
10 on H
2O
2-induced SIPS model of human skin fibroblasts has not been understood. So that, we investigated that human skin fibroblasts were used to investigate the prevention effect of CoQ
10 against H
2O
2-induced SIPS model. We created SIPS model fibroblasts with treatment of 100 μM H
2O
2 for 2 h. In this study, CoQ
10 also increased cell viability and mRNA levels of type I, IV collagen and protein level of type I collagen. Moreover, it is shown that CoQ
10 suppressed oxidative stress, degradation of collagen by increasing MMP expression, and decreasing senescence-associated phenotypes (e.g. SA-βgal positive staining and SASP) for preventing skin aging via H
2O
2-induced SIPS model. These results suggested that CoQ
10 has possibility to be contributory for extension of healthy life expectancy in Japan.
