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  • 标题:Author Correction: Determinants of duck Tembusu virus NS2A/2B polyprotein procession attenuated viral replication and proliferation in vitro
  • 本地全文:下载
  • 作者:Bowen Jiang ; Wei Zhang ; Yuanyuan Wu
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2021
  • 卷号:11
  • DOI:10.1038/s41598-021-03536-w
  • 语种:English
  • 出版社:Springer Nature
  • 摘要:Correction to: Scientifc Reports 10.1038/s41598-020-68271-0, published online 24 July 2020 The original version of this Article contained an error in Figure 1D where the label indicating “NS2B-Flag(14kDa)” was incorrectly given as “Myc-NS2B(25kDa).” The original Figure  1 and accompanying legend appear below. Figure 1 Cleavage of NS2A/2B by NS2B3. ( A) Genome structure and cleavage sites of flavivirus polyprotein. ( B) Conservative amino acid residues near the NS2A/2B cleavage site by comparison of different flaviviruses, including DENV, JEV WNV, YFV, TBEV, BGAV, ZIKA and KUN. (C) Overexpression of DTMUV NS2A/2B in transfected DEFs. DEF cells were transfected with different concentrations of pCAGGS-Myc-NS2A/2B-Flag and the cells were harvested 24 h post transfection. ( D, E) Cleavage of NS2A/2B by NS2B3. DEF cells were cotransfected with plasmids expressing NS2A/2B and with different concentrations of NS2B3 plasmids, and proteins of interest were detected by WB 24 h post transfection. ( D) Mouse anti-Flag monoclonal antibody was used as the primary antibodies, ( E) Mouse anti-Myc monoclonal antibody was used as the primary antibody. The original Article has been corrected.
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