摘要：CNGCs are ligand-gated calcium signaling channels, which participate in important biological processes in eukaryotes. However, the
CNGC gene family is not well-investigated in
Brassica rapa L. (i.e., field mustard) that is economically important and evolutionary model crop. In this study, we systematically identified 29 member genes in
BrCNGC gene family, and studied their physico-chemical properties. The
BrCNGC family was classified into four major and two sub phylogenetic groups. These genes were randomly localized on nine chromosomes, and dispersed into three sub-genomes of
B. rapa L. Both whole-genome triplication and gene duplication (i.e., segmental/tandem) events participated in the expansion of the
BrCNGC family. Using
in-silico bioinformatics approaches, we determined the gene structures, conserved motif compositions, protein interaction networks, and revealed that most
BrCNGCs can be regulated by phosphorylation and microRNAs of diverse functionality. The differential expression patterns of
BrCNGC genes in different plant tissues, and in response to different biotic, abiotic and hormonal stress types, suggest their strong role in plant growth, development and stress tolerance. Notably,
BrCNGC-9,
27,
18 and
11 exhibited highest responses in terms of fold-changes against club-root pathogen
Plasmodiophora brassicae,
Pseudomonas syringae pv.
maculicola, methyl-jasmonate, and trace elements. These results provide foundation for the selection of candidate
BrCNGC genes for future breeding of field mustard.
