期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2021
卷号：118
期号：47
DOI：10.1073/pnas.2110767118
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Significance
In animals, the control of daily sleep–wake rhythms is mediated by discrete circadian clock neurons via their rhythmic activity–dependent release of neuropeptides and neurotransmitters. Here, we describe a gene,
Tango10, critical for daily behavioral rhythms. TANGO10 functions as an adaptor with its partner, the E3 ubiquitin ligase CULLIN 3, to regulate protein ubiquitination, neuronal excitability via voltage-gated potassium channels, and neuropeptide accumulation. These studies define a mechanism for control of neuronal excitability and daily rhythmicity.
Circadian transcriptional timekeepers in pacemaker neurons drive profound daily rhythms in sleep and wake. Here we reveal a molecular pathway that links core transcriptional oscillators to neuronal and behavioral rhythms. Using two independent genetic screens, we identified mutants of
Transport and Golgi organization 10 (
Tango10) with poor behavioral rhythmicity.
Tango10 expression in pacemaker neurons expressing the neuropeptide PIGMENT-DISPERSING FACTOR (PDF) is required for robust rhythms. Loss of
Tango10 results in elevated PDF accumulation in nerve terminals even in mutants lacking a functional core clock. TANGO10 protein itself is rhythmically expressed in PDF terminals. Mass spectrometry of TANGO10 complexes reveals interactions with the E3 ubiquitin ligase CULLIN 3 (CUL3). CUL3 depletion phenocopies
Tango10 mutant effects on PDF even in the absence of the core clock gene
timeless. Patch clamp electrophysiology in
Tango10 mutant neurons demonstrates elevated spontaneous firing potentially due to reduced voltage-gated Shaker-like potassium currents. We propose that
Tango10/Cul3 transduces molecular oscillations from the core clock to neuropeptide release important for behavioral rhythms.
关键词：circadian rhythms; neuronal output; ubiquitin ligase; Drosophila; potassium current
