摘要：Rapeseed, a major oil crop in the world, is easily affected by low-temperature stress. A low temperature delays seed germination and increases seedling mortality, adversely affecting rapeseed growth and production. In the present study, a tolerant cultivar (Huyou21) was crossed with a susceptible genotype (3429) to develop a mapping population consisting of 574 F
2 progenies and elucidate the genetic mechanisms of seed germination under low temperatures. Two quantitative trait loci (QTL) for low-temperature germination (LTG) were detected, one on chromosome A09 (named
qLTGA9-1) and the other on chromosome C01 (named
qLTGC1-1), using the QTL-seq approach and confirmed via linkage analysis in the mapping population. Further,
qLTGA9-1 was mapped to a 341.86 kb interval between the SSR markers
Nys9A212 and
Nys9A215. In this region, 69 genes including six specific genes with moderate or high effect function variants were identified based on the Ningyou7 genome sequence. Meanwhile,
qLTGC1-1 was mapped onto a 1.31 Mb interval between SSR markers
Nys1C96 and
Nys1C117. In this region, 133 genes including five specific genes with moderate effect function variants were identified. These specific genes within the two QTL could be used for further studies on cold tolerance and as targets in rapeseed breeding programs.
