摘要：AbstractChemotactic responses play a significant role duringLeishmania (V.) braziliensisdifferentiation through its life cycle and during infection. The aim of this description has been to portray the modified “two-chamber capillary chemotaxis assay” as a technique useful for quantitativein vitroevaluation ofLeishmaniachemotaxis after reviewing the methods described until now to assess chemotaxisin vitroinLeishmania sp. This valued simple and reproducible method convenient for parasite migration determination, was tested by the use of controlled changes in monosaccharide (D-glucose and D-fructose) concentrations as referent ligands. The validation of the method demonstrates that this technique is useful to evaluate the relationship existing between parasite migration towards the monosaccharides and sugar concentration. This means that within specific ranges, parasites attracted by the monosaccharide migrate towards more concentrated solutions and accumulate (higher number of parasites) at that spot. Interestingly, both the time course of the experiment and the osmolality of the solution influence parasite migration capacity. Our validation suggests that this improved methodology quantitatively evaluates taxis ofLeishmaniatowards/against different substances. On the basis of our herein presented data, we conclude that this technique is a novel, rapid and reliable screening method to evaluate chemotaxis inLeishmania.•The two-chamber capillary chemotaxis assay was standardized forLeishmania.•The technique is useful to quantitatively evaluate in vitro chemotaxis inLeishmania.•Parasite migration was characterized by monosaccharide chemical gradients.•This assay is a novel, rapid and reliable screening method to evaluate chemotaxis.Contain between 1 and 3 bullet points highlighting the customization rather than the steps of the procedure.Graphical abstractDisplay Omitted
