摘要：Pectin was extracted from tobacco stems using an HCl-CH3COOH mixed acid extraction method to assess as a potential tool in commercial pectin pro-duction. The effect of hot and cold pretreatments on the pectin yield was investigated first. Then, the re-sponse surface methodology was used to determine the effects of the solid-liquid ratio(1/20-1/40), ex-traction temperature(80-100 ℃C) and time(90-150 min) on the pectin yield, and the maximum extrac-tion yield appeared at 88.8℃C,128 min and a solid-liquid ratio of 1/34.Lastly, the pectins obtained from the mixed acid and single acid treatments were ana-lyzed and compared by yield, structure and physico-chemical properties. According to the results, it can be concluded that the mixed HCl-CH;COOH acid could combine the yield and esterification degree of strong inorganic acid and organic acid and yield pec-tin products with ideal physicochemical properties.
