摘要：The present study was performed to determine the effects of different genotypes and concentrations of sodium azide on callus formation and plant regen- eration from endosperm supported mature embryo derived calli of barley. Callus formation of endo- sperm supported mature embryos of two barley gen- otype were cultured in MS medium with five differ-ent concentrations of sodium azide (NaN) (0, 10-4. 10-3, 10-2, and 10-1 mM). Results revealed that the main effects of both genotypes, sodium azide con- centrations and their interactions on callus and em- briogenic callus development and regeneration were significant or highly significant, suggesting that gen- otypes responded differently to the different sodium azide concentrations. The application of sodium az- ide application decreased the callus induction rate. embryogenic callus rate, responded embryogenic callus rate and regeneration efficiency. When evalu- ating the concentrations based on the averages, the highest regeneration efficiency and responded em- bryogenic callus rate were obtained at 10-1 mM and 104 mM concentrations, respectively, compared with the control. The highest callus induction rate (81.12%) in Akar genotype and the highest regener- ation efficiency (1.99 plants) were observed in O1- gun genotype.
关键词：Callus;chemical mutagens;in vitro;plant regeneration;tis-
sue culture
