摘要：Insects and other biological pests devastate economically important crops. Synthetic pesticides applied carelessly to treat insect pests have negative consequences for the plant, livestock, human health, and ecosystem apart from acquired resistance. In re-cent years, botanical crude extracts and bioactive secondary metabolites as a pesticide gained a lot of attention as an alternative eco-friendly approach for the pest management program. This study was aimed to check the efficacy of a naturally occurring bioac-tive polyphenolic compound rosmarinic acid (RA)against laboratory-reared insects;Lesser grain borer (Rhnyzopertha dominica L.),Citrus mealybug (Planococcus citri),and Peach fruit fly(Bactrocera dorsalis L.)by ingestion bioassay as a potential can-didate for botanical insecticide. The results indicated that RA showed significant toxicity in R. domimnica L.and adult B.dorsalis, with no mortality in P.citri L. RA, showed maximum toxicity against R. domi-nica L.in ingestion bioassay with an LCso estimate value of 42.41 ug/mL followed by adult bioassay of B. dorsalis (male and female) with LCso estimate values of, 9.1l and 23.55 ug/mL at 24 and 48 hours after treatment, respectively. These major findings suggest that RA has considerable promise as a bo-tanical pesticide and can be used to protect crops as a strong and safer agent against a variety of insects. Future research should concentrate on the determi-nation of its mode of action by enzyme inhibition bi-oassays so that it can be adopted as an insecticide against the targeted insects in agricultural habitats.
关键词：Bioactive Compound;Rosmarinic acid;Bioassay;LCs;Toxicity;Peach fruit fly
