摘要：To analyze main factors affecting the quality of microbiological examination and common drug re- sistance of pathogens. A retrospective survey was carried out on 8974 microbial test samples. The accuracy of sample cul-tivation, number and composition of pathogenic bac- teria were collected. The factors influencing the quality of microbi- ological test include: operation level of laboratory personnel, quality of instruments and equipment, physiological status of patients, methods of speci- men collection and detection methods. There were 1723 specimens of pathogenic bacteria detected from all samples, and the positive rate was 19.20%; there were 1572 pieces of pathogenic bacteria checked out, distributed mainly in sputum specimens (49.30%), urine specimens (15.97%), and bloodsamples (9.92%). The pathogenic bacteria were gram-negative bacteria (51.65%), gram-positive bacteria (39.76%) and fungus (8.59%), among which staphylococcus aureus, escherichia coli and klebsiella pneumoniae took up the largest part. The drug resistance rate of escherichia coli and klebsiella pneumoniae to quinolones and cephalosporins washigher than 50.00%. The drug resistance rate of staphylococcus aureus to quinolones, erythromycin and lactams was over 50.00%. The quality of microbiological examination will be enhanced by improving operating level of clinical laboratory personnel, standardizing testing process and improving the equipment and quality of specimens.
关键词：Microbial test;clinical laboratory;pathogenic bacteria
drug resistance;rational use of drugs
