摘要：The commercialization of microalgae biofuel is being inhibited by significant drawbacks such as low lipid productivity and the high cost of feedstock. As such, extensive research efforts have focused on im-proving cultivation strategies to achieve high cell densities while maximizing lipid content. Therefore, the purpose of this study was to evaluate the effects of three main growth factors—-temperature, indole-3-propionic acid (IPA), and ammonium-on the growth rate, biomass density, and lipid productivity of Chlorella pyrenoidosa. The Fourier transform in-frared spectroscopic data collected in this study were used to build multivariate calibration models. The re-sults showed that ammonium and IPA concentrations of60 mg L-1 and 252.5 mg L-1,respectively, were optimal for algal growth at 30℃C.Box-Behnken de-sign response surface methodology indicated that under optimal temperature and nitrogen concentra-tions, a relatively low concentration of IPA could stimulate the growth ofChlorella. Meanwhile,a high nitrogen concentration could serve as stress to in-duce lipid accumulation. The toxic effect of exces-sive ammonium might be alleviated by the supple-ment of IPA in the medium, enabling the cells to grow on excess ammonium.
