摘要：This study was carried out to determine the an-tifungal activity of mint(Mentha piperita L.),thyme (Thymus vulgaris L.)and eucalyptus(Eucalyptus cit-riodora Hook.)essential oils against 4 different pathotypes of chickpea blight(Ascochytarabiei) un-der in-vitro conditions. The antifungal activity assay was performed in 100 mm petri dishes containing 20 mL Potato-Dextrose-Agar (PDA)media. Essential oils were added to petri dishescontaining autoclaved PDA medium in doses of 0.125,0.25,0.5,1 and 2 uL mL-.5 mm diameter fungal discs were placed in the middle of petri dishes. PDA medium free of es-sential oils as negative control and as a positive con-trol PDA media containing commercial fungi-cide %25.2 Boscalid+%12.8 Pyraclostrobin(BASF company)were used. Petri dishes sealed with para-film were incubated for 14 days at 22±1°C.The ex-periment was conducted in a randomized plot design with threereplications. Measurements were made af-ter completion of the incubation period and percent inhibition of mycelial growth was determined. It was determined that mint essential oil completely inhib-ited mycelium growth of pathotype I, II and IV, by 100% and the effect was fungicidal at the positive control.The dose of 2 uL mL-and positive control inhibited mycelium growth of pathotype II,by 81.3%and 82.9%respectively. It was found that thyme es-sential oil completely inhibited mycelium growth of all pathotypes at the dose of2μL mL- and positive control inhibited mycelium growth of pathotypes I and ⅡII, by 100% and the effects was fungicidal. It was seen that eucalyptus essential oil completely in-hibited mycelium growth of pathotypes II and IV, by 100% and this effect was fungicidal at the positive control. The dose of 2uL mL-and positive control had an effect of 84.9% and 84.1% against pathotype I,84.1%and 83.7% against pathotype II, respec-tively.
关键词：Ascochyta rabiei;essential oils;antifungal activity;chick-pea;alternative control
