标题：EFFECTS OF PHYSICOCHEMICALFACTORS ON THE NITROGEN TRANSFORMATION FUNCTIONAL GENES OF MICROORGANISMS DURING THE INTERMITTENT AERATION IN POLLUTED RIVER
摘要：The nitrogen transformation functional genes in microbes are the basis of biological nitrogen re-moval.It is significant to study the effect ofphysico-chemical factors in sediment on nitrogen transfor-mation genes during the biological nitrogen removal. The fluorescence quantitative PCR was used to de-tect the abundance of nitrogen transformation genes. The redundancy analysis and the variation partition-ing analysis were used to analyze the physicochemi-cal factors affecting nitrogen transformation func-tion genes. The abundance of nitrogen transfor-mation functional genes showed that the short-cut bi-ological nitrogen removal was achieved with the in-termittent aeration method. During the short-cut ni-trification stage, the ammonia monooxygenase (amoA)gene had an obvious growth trend, and that there was no significant change in the nitrous oxide reductase (nxr)gene, which indicated that ammonia nitrogen was mainly converted into nitrite nitrogen. During the short-cut denitrification stage, the copper type nitrite reductase gene (nirK) and the cyto-chrome nitrite reductase (nirS)genes increased sig-nificantly, but the nitrate reductase (narG)gene did not change much, which indicated that the transfor-mation of nitrite nitrogen to nitric oxide is the main reaction in short-cut denitrification. Redundancy analysis showed that, during the short-cut nitrifica-tion stage, the amoA gene was positively correlated with the dissolved oxygen and the ammonia nitrogen;During the short-cut denitrification stage, the nirK gene, the nirS gene, the nitrous oxide reductase (nosZ) gene, the hydrazine synthase(hzsA, hzsB)gene and the hydrazine oxidase (Hzo)gene were negatively correlated with the dissolved oxygen. The results of the variance partitioning analysis showed that, during the short-cut nitrification stage, the dis-solved oxygen, the ammonia nitrogen and the C:N (carbon to nitrogen ratio)were the main factors af-fecting the nitrogen transformation functional genes. However, the dissolved oxygen, the nitrite nitrogen and the total organic carbon were the main factors affecting the nitrogen transformation functional genes.
关键词：Nitrogen transformation function gene;intermittent aera-tion;physical and chemical factors;redundancy analysis
