摘要：In the present study, an attempt has been made to analyze the toxicity of fluoride and arsenic on morphology and behaviour of fish Clarias batrachus. Based on 96 hour LC50 values at the 95% confidence limits of sodium fluoride (NaF) and arsenic trioxide (As2O3) obtained from the preliminary tests, various sub-lethal concentrations of NaF and As2O3 were selected for combined toxicity testing. During the experiment, fish were regularly observed for any changes in their external morphology and behaviour. Behavioural changes were observed in terms of air gulping, opercular movement, swimming activity, body position, general activity and food sensitivity. Control fish remained normal throughout the experiment period (96 hour) but erratic, exodus and speedy movements were found in all treated groups. The number of air gulps (per 15 min, 24-96 hour) significantly increased (P<0.001) in the exposed Group I (20 mg.L-1 As2O3) as compared to control. A significant increase (P<0.01) in the number of opercular movements (per min for 24-96 hour) was recorded in treatment groups as compared to control. Apparent changes such as excessive mucous secretion, increased body discolouration, loosening of skin and complete loss of skin (head region and fins) were also observed. Skin loss was more intense in fish subjected to Group I.
