标题：Effect of Temperature Downshifts on Performance and Microbial Community Structure on Pilot-Scale Sequencing Batch Biofilm Reactors Treating Hypersaline Wastewater
摘要：Low temperature and high salinity can strongly inhibit metabolic activities of the microbial population, resulting in low efficiency of biological wastewater treatment. Using 70 g.L-1 NaCl pickle mustard wastewater as influent, three pilot-scale sequencing batch biofilm reactors (SBBRs), subjected to temperature downshifts and fluctuation ranging from 30 ± 4°C to 10 ± 4°C, were conducted over 200 days. Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR–DGGE) was used to reveal the microbial community structure succession in reactors. Results showed that when the temperature was 10 ± 4°C, the COD removal efficiencies of SBBRs (1, 2 and 3 kg COD m-3 d-1organic loading rate), were 91.6% (σ = 0.87), 87.84% (σ = 0.92) and 83.34 % (σ = 0.85), respectively. Compared with the average removal efficiencies when the reactors operated at 30 ± 4°C, the efficiency reductions of 1, 2 and 3 kg COD m-3 d-1 reactors were 4.47%, 4.58% and 4.57%. As the temperature decreased, microbial population diversity did not change remarkably. However, the microbial structure changed significantly, bacteria which had strong adhesion and a wide growth temperature range were competitive. At low temperature, the predominant species were Thalassolituus oleivorans, Halotalea alkalilenta and Kangiella koreensis, which were all related to pollutant-degrading halophilic bacteria.
关键词：Pilot-scale SBBR;Hypersaline wastewater;PCR-DGGE;Bacterial community
