摘要：AbstractThis study investigates the co-cultivation system ofNitzschia palea(FACHB-2263) andPseudomonas chlororaphis(ACCC-19853) in the artificial consortium at different concentrations. The algicidal mode is evaluated using three different full factorial experiments (FFEs) under the following three scenarios: different bacteria culture cell densities against different diatoms; algicidal activities of bacterial culture supernatant against theN. paleain different growth phases, such as lag, exponential and stationary phase; different bacteria and diatom against artificial consortium at different concentrations. For minimizing the effect of random error, three replications of each experimental run of these three FFEs are used. The influence of bacteria on growth in total chlorophyll is analyzed for all of these three replicates FFEs. The results demonstrate that: theP. chlororaphisshowed high algicidal activity againstN. Paleawithin 5 days; selected bacteria culture supernatant exhibited strong algicidal activity in the lag phase while the artificial consortium achieved the algal cell lysis at different concentrations of the bacteria to the diatom cells and the diatom to the bacteria cell; and the indoor mesocosms confirmed thatP. chlororaphiseffectively reducedN. palea cells by over 90% within 5 days. The findings suggest that the artificial consortia algal-bacterial symbiotic system is thus proven to be an effective and promising system for microalgae bloom control treatment algal-bacterial symbiotic system that was extrapolated for novel algae bloom control treatment.
