摘要：In this study, thymoquinone (TQ), a natural active substance, was investigated for its antibacterial activity against
Bacillus cereus, and its inhibitory effect on
B. cereus in reconstituted infant formula (RIF) was evaluated. In addition, the inhibitory effect of TQ on
B. cereus spore germination was explored. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of TQ against eight
B. cereus strains ranged from 4.0 to 8.0 μg/mL, whereas
B. cereus treated with TQ displayed a longer lag phase than the untreated control. TQ exerted a good bactericidal effect on
B. cereus in Luria–Bertani broth. In addition, TQ obviously reduced the intracellular ATP concentration of
B. cereus, which caused depolarization of the cell membrane, increased the intracellular reactive oxygen species level, impaired the cell morphology, and destroyed proteins or inhibited proteins synthesis. This provides a mechanism for its bacteriostatic effect. TQ also inactivated
B. cereus growth in RIF. Moreover, reverse transcription–quantitative polymerase chain reaction illustrated that TQ downregulated the transcription of genes related to hemolysin, non-hemolytic enterotoxin, enterotoxin, and cytotoxin K. Meanwhile, TQ displayed the ability to inhibit the germination of
B. cereus spores. These findings indicate that TQ, as an effective natural antimicrobial preservative, has potential applications in controlling food contamination and foodborne diseases caused by
B. cereus.
